來了來了！四季度客戶文獻(xiàn)精選集錦來啦
四季度共收集引用Genomeditech Co.,Ltd. (Shangha,China)文章共計(jì)96篇，影響因子(IF)超500分。其中包括Cancer Cell以及Nucleic Acids Research，Advanced Science，Nature Communications影響因子15+以上的文章8篇。
文章所涵蓋的產(chǎn)品和服務(wù)包含常規(guī)質(zhì)粒構(gòu)建、慢病毒、腺相關(guān)病毒、細(xì)胞株構(gòu)建、活體成像、熒光素酶報(bào)告基因，還包含了自噬，circRNA，CRISPR/Cas9技術(shù)等。
本篇精選了四季度新發(fā)的部分高質(zhì)量文章，讓我們一起追蹤最新科研進(jìn)展！
細(xì)胞產(chǎn)品篇
1、多組學(xué)綜合圖譜解析轉(zhuǎn)移性肝癌的時(shí)空演變
文獻(xiàn)標(biāo)題：Integrated multi-omics profiling to dissect the spatiotemporal evolution of metastatic hepatocellular carcinoma
影響因子：50.3
發(fā)表期刊：Cancer Cell
發(fā)表單位：復(fù)旦大學(xué)附屬中山醫(yī)院
摘要：目前肝癌轉(zhuǎn)移腫瘤的基因突變和分子表達(dá)全景圖譜仍極度缺乏，肝癌在轉(zhuǎn)移過程中的克隆選擇等演化規(guī)律尚不清晰，導(dǎo)致肝癌轉(zhuǎn)移病灶的臨床診療仍然缺乏足夠的理論指導(dǎo)。在這里，作者對(duì)182例HCC患者的257個(gè)原發(fā)性和176個(gè)轉(zhuǎn)移灶進(jìn)行了多組分析。原發(fā)性和轉(zhuǎn)移性HCC的基因組差異很高，早期播散很普遍。在轉(zhuǎn)移瘤中觀察到的顯著的腫瘤內(nèi)新抗原異質(zhì)性與T細(xì)胞反應(yīng)性降低有關(guān)，這是由于新抗原遞呈中斷所致。沒有Wnt突變的亞克隆比有Wnt突變的亞克隆顯示出更強(qiáng)的轉(zhuǎn)移選擇性優(yōu)勢，并以微環(huán)境中豐富的活化成纖維細(xì)胞為特征，有利于前轉(zhuǎn)移表型。最后，沒有Wnt突變的轉(zhuǎn)移瘤表現(xiàn)出更高的免疫抑制B細(xì)胞富集，通過HLA-E:CD94-NKG2A檢查點(diǎn)軸介導(dǎo)CD8+ T細(xì)胞的末端衰竭?？偟膩碚f，作者的結(jié)果提供了一個(gè)復(fù)雜的轉(zhuǎn)移進(jìn)化過程的多維解剖。
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AXIN1敲除單克隆細(xì)胞系：AXIN1-knockout MHCC97H cell line
干擾慢病毒：PGMLV-hU6-sh-HIF1a-CMV-Blasticidin-WPRE
過表達(dá)慢病毒：PGMLV-CMV-HIF1a-33Flag-PGK-Blasticidin
2、Claudin18.2/4–1BB 雙特異性抗體- 792 Givastomig 在 1L/2L 胃癌治療中發(fā)揮旁觀者腫瘤殺傷和協(xié)同抗腫瘤活性
文獻(xiàn)標(biāo)題：792 Givastomig, a novel Claudin18.2/4–1BB bispecific antibody, exerts bystander tumor-killing and synergistic anti-tumor activity with therapeutics in 1L/2L treatment for gastric cancer
影響因子：10.9
發(fā)表期刊：Journal for Immunotherapy of Cancer
發(fā)表單位：天境生物制藥
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CLDN18.2過表達(dá)細(xì)胞株
原文引用：The CLDN18.2 expression in formalin-fixed,paraffin-embedded(FFPE) tumors of three human gastric cancer cell lines (MKN-45,MKN-45#14,and MKN-45#18; obtained from Genomeditech)
3、一種新型介孔二氧化硅納米系統(tǒng)為治療SARS-CoV-2感染提供了新的可能
文獻(xiàn)標(biāo)題：A coronavirus-mimic mesoporous silica nanosystem enables efficient targeted delivery of siRNA for anti-SARS-CoV‐2
影響因子：8.3
發(fā)表期刊：Applied Materials Today
發(fā)表單位：中國科學(xué)院上海硅酸鹽研究所
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ACE2 OE-293T過表達(dá)細(xì)胞株
原文引用：In addition, the RdRp gene of the SARS-CoV-2 genome (MT039890) was stably transfected into the ACE2 OE-293T cells by a lentivirus infection method (Genomeditech)
Luciferase產(chǎn)品篇
4、腫瘤內(nèi)真菌群落通過髓源性抑制細(xì)胞促進(jìn)肺癌進(jìn)展
文獻(xiàn)標(biāo)題：Histone lactylation-boosted ALKBH3 potentiates tumor progression and diminished promyelocytic leukemia protein nuclear condensates by m1A demethylation of SP100A
影響因子：19.2
發(fā)表期刊：Nucleic Acids Research
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院
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ALKBH3/SP100A過表達(dá)和干擾慢病毒質(zhì)粒
原文引用：The PCMLW-CMV-MCS-EF1-ZsGreen1-T2A-Puro,CMLV-hU6-MCS-CMV-ZsGreen1-PCK-Puro WPRE,ZV502 and ZV102 vectors were used in our study. ALKBH3 shRNAs，SP100A shRNA,YTHDF1 shRNA and their corresponding verified negative control sequences were amplified by PCR and inserted into the PCMLV-hU6-MCS-CMV-ZsGreen1-PGK-Puro-WPRE vectors. The SP100A ORF was generated by PCR and cloned into the PCMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro vector.
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m1A甲基化；SP100A-5UTR報(bào)告基因質(zhì)粒
原文引用：The DNA fragments of the SP100A-5UTR containing the wild-type m1A motifs and mutant motifs (potential m A was replaced by T) were synthesized and inserted upstream of firefly luciferase of the pmirCLO vector.
5、?；撬嵋种魄傲邢侔┠[瘤細(xì)胞和腫瘤相關(guān)巨噬細(xì)胞之間的串?dāng)_介導(dǎo)的鐵死亡
文獻(xiàn)標(biāo)題：Taurine Inhibits Ferroptosis Mediated by the Crosstalk between Tumor Cells and Tumor-Associated Macrophages in Prostate Cancer
影響因子：15.1
發(fā)表期刊：Advanced Science
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院
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lats1 mRNA 3′-UTR報(bào)告基因質(zhì)粒
原文引用：For the dual luciferase assay, wild type (WT) or mutant (MT) putative miR-181a-5p binding site sequences on lats1 mRNA 3′-UTR region were purchased from Genomeditech
質(zhì)粒和慢病毒產(chǎn)品篇
6、BPTF 通過表觀遺傳調(diào)控 C-MYC/PLCG1/Perk 軸來驅(qū)動(dòng)胃癌對(duì) EGFR 抑制劑的耐藥性
文獻(xiàn)標(biāo)題：BPTF Drives Gastric Cancer Resistance to EGFR Inhibitor by Epigenetically Regulating the C-MYC/PLCG1/Perk Axis
影響因子：15.1
發(fā)表期刊：Advanced Science
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院
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PLCG1過表達(dá)和siRNA
原文引用：Overexpression and Knockdown of PLCG1 and c-MYC Genes: Control and PLCG1/c-MYC siRNAs (PLCG1, GAGTTTGTGGGTCAGTCTG, cMYC, GCTTCACCAACAGGAACTA) were purchased from Genomeditech(Shanghai, China)
7、錳通過靶向 T 淋巴細(xì)胞極化增強(qiáng)硅羥基磷灰石納米線的成骨作用
文獻(xiàn)標(biāo)題：Manganese Enhances the Osteogenic Effect of Silicon-Hydroxyapatite Nanowires by Targeting T Lymphocyte Polarization
影響因子：15.1
發(fā)表期刊：Advanced Science
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院
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小鼠Sod2干擾慢病毒
原文引用：The MnSOD shRNA lentivirus was constructed by Genomeditech(Shanghai, China).
8、富馬酸二甲酯（DMF）在改善雙側(cè)海綿體神經(jīng)損傷（BCNI）大鼠勃起功能方面的治療潛力
文獻(xiàn)標(biāo)題：Dimethyl fumarate ameliorates erectile dysfunction in bilateral cavernous nerve injury rats by inhibiting oxidative stress and NLRP3 inflammasome-mediated pyroptosis of nerve via activation of Nrf2/HO-1 signaling pathway
影響因子：11.4
發(fā)表期刊：Redox Biology
發(fā)表單位：華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院
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大鼠Nfe2l2、Ho-1干擾慢病毒質(zhì)粒
原文引用：The lentiviral vectors were generated and the competent cells containing target plasmids were constructed by Genomeditech
9、非ACE2依賴SARS-CoV-2病毒通過單核細(xì)胞表面GRP78感染
文獻(xiàn)標(biāo)題：ACE2-independent SARS-CoV-2 virus entry through cell surface GRP78 on monocytes -evidence from a translational clinical and experimental approach
影響因子：11.1
發(fā)表期刊：EBioMedicine
發(fā)表單位：華中科技大學(xué)同濟(jì)醫(yī)學(xué)院
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GRP78 過表達(dá)質(zhì)粒和慢病毒；HG轉(zhuǎn)染試劑
原文引用：To establish overexpression of GRP78 in THP-1 cells, human HSPA5 gene segment was inserted into PGMLV-CMV-MCS-EF1-mScarlet-T2A-Puro plasmid (Genomeditech, Shanghai, China). hGRP78 over-expressed plasmid or mock control plasmid were co-transfected with lentivirus-packaging plasmid (Genomeditech, Shanghai, China) into HEK293T cells using HG transgene reagent (Genomeditech, Shanghai, China) according to the manufacturer's protocol.
腺相關(guān)病毒產(chǎn)品篇
10、一種新型酞嗪酮衍生物作為衣殼組裝調(diào)節(jié)劑抑制乙型肝炎病毒表達(dá)
文獻(xiàn)標(biāo)題：A novel phthalazinone derivative as a capsid assembly modulator inhibits hepatitis B virus expression
影響因子：7.6
發(fā)表期刊：Antiviral Research
發(fā)表單位：中國科學(xué)院上海藥物研究所
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HBV（AYW）腺相關(guān)病毒
原文引用：Adeno-associated virus (capsid serotype AAV8) expressing a 1.1-mer overlength HBV genome (serotype ayw) was purchased from Genomeditech (Shanghai, China)
11、METTL3通過促進(jìn)TRAF6依賴性線粒體ROS產(chǎn)生導(dǎo)致MI后交感神經(jīng)亢進(jìn)
文獻(xiàn)標(biāo)題：m6A methyltransferase METTL3 contributes to sympathetic hyperactivity post-MI via promoting TRAF6-dependent mitochondrial ROS production
影響因子：7.4
發(fā)表期刊：Free Radical Biology and Medicine
發(fā)表單位：山東第一醫(yī)科大學(xué)附屬第一醫(yī)院
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ACE2 OE-293T過表達(dá)細(xì)胞株
原文引用：In addition, the RdRp gene of the SARS-CoV-2 genome (MT039890) was stably transfected into the ACE2 OE-293T cells by a lentivirus infection method (Genomeditech)
現(xiàn)貨產(chǎn)品篇
12、LncRNA INHEG 通過調(diào)節(jié) rRNA 2'-O-甲基化促進(jìn)膠質(zhì)瘤干細(xì)胞的維持和致瘤性
文獻(xiàn)標(biāo)題：LncRNA INHEG promotes glioma stem cell maintenance and tumorigenicity through regulating rRNA 2’-O-methylation
影響因子：16.6
發(fā)表期刊：Nature Communications
發(fā)表單位：中國科學(xué)院生物物理研究所
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慢病毒濃縮試劑盒
原文引用：Lentiviral particles were collected 48 h after media change and concentrated using the lentivirus concentrated kit (Genomeditech) according to the manufacturer’s instructions
13、一種抗體調(diào)理作用的免疫代謝 NP (immeNPs)，用于腫瘤浸潤單核吞噬的靶向雙重干預(yù)
文獻(xiàn)標(biāo)題：Reshaping Intratumoral Mononuclear Phagocytes with Antibody-Opsonized Immunometabolic Nanoparticles
影響因子：15.1004
發(fā)表期刊：Advanced Science
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院
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D-Luciferin(150μg?1,Genomeditech, GM040611) 熒光素鈉鹽
