最新！一季度客戶文獻(xiàn)精選集錦來(lái)啦
2024年一季度共收集引用Genomeditech Co.,Ltd. (Shangha,China)文章共計(jì)95篇，影響因子(IF)近680分。其中包括Cell、Cell Metabolism、Nano Today、Nature communications、Advanced Science影響因子15+以上的文章8篇。
文章所涵蓋的產(chǎn)品和服務(wù)包含常規(guī)質(zhì)粒構(gòu)建、慢病毒、腺相關(guān)病毒、細(xì)胞株構(gòu)建、活體成像、熒光素酶報(bào)告基因，還包含了轉(zhuǎn)染試劑，慢病毒包裝試劑盒，siRNA等常用科研產(chǎn)品類試劑。
本篇精選了一季度新發(fā)的部分高質(zhì)量文章，讓我們一起追蹤最新科研進(jìn)展！
質(zhì)粒產(chǎn)品篇
1、中性粒細(xì)胞圖譜闡明其抗腫瘤過(guò)程中的抗原呈遞作用
文獻(xiàn)標(biāo)題：Neutrophil profiling illuminates anti-tumor antigen-presenting potency
影響因子：64.5
發(fā)表期刊：Cell
發(fā)表單位：復(fù)旦大學(xué)附屬中山醫(yī)院
摘要：中性粒細(xì)胞是抵御病原體最豐富和最有效的防御者，在不同癌癥類型中發(fā)揮相反的功能。然而，由于它們的半衰期較短，探索中性粒細(xì)胞如何在癌癥中采取特定的命運(yùn)仍然具有挑戰(zhàn)性。在這里，作者從17種癌癥類型（來(lái)自143例患者的225個(gè)樣本）中生成并整合了單細(xì)胞中性粒細(xì)胞轉(zhuǎn)錄組。中性粒細(xì)胞表現(xiàn)出異常的復(fù)雜性，有10種不同的狀態(tài)，包括炎癥、血管生成和抗原呈遞。值得注意的是，在大多數(shù)癌癥中，抗原-呈遞程序與良好的生存率相關(guān)，并可通過(guò)亮氨酸代謝和隨后的組蛋白H3K27ac修飾引起。這些中性粒細(xì)胞可以進(jìn)一步引起（neo）抗原特異性和抗原非依賴性T細(xì)胞反應(yīng)。中性粒細(xì)胞傳遞或亮氨酸飲食微調(diào)免疫平衡，以增強(qiáng)抗PD-1治療?？傊@些數(shù)據(jù)不僅表明了不同癌癥間中性粒細(xì)胞的差異，而且還提示了治療機(jī)會(huì)，如抗原呈遞中性粒細(xì)胞傳遞。
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Human: dHL-60 cells. 人早幼粒白血病細(xì)胞
RFX5 過(guò)表達(dá)和干擾質(zhì)粒
2、組蛋白乙酰轉(zhuǎn)移酶6A介導(dǎo)CD4+T細(xì)胞糖代謝重編程影響自身免疫疾病
文獻(xiàn)標(biāo)題：Lysine acetyltransferase 6A maintains CD4+ T cell response via epigenetic reprogramming of glucose metabolism in autoimmunity
影響因子：28.9
發(fā)表期刊：Cell Metabolism
發(fā)表單位：上海市第九人民醫(yī)院
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KAT6A 敲除質(zhì)粒
原文引用：The CRISPR/Cas9 system was used to delete the genomic DNA of mouse KAT6A ex vivo. sgRNAs targeting mouse KAT6A and nontargeting control (mouse) sgRNA were designed (Table S4), cloned and inserted into a retro-gRNA-eGFP vector. gRNA was synthesized by Genomeditech Corp. (Shanghai, China).
3、Cullin5通過(guò)調(diào)節(jié)肺泡巨噬細(xì)胞抗病毒免疫來(lái)驅(qū)動(dòng)實(shí)驗(yàn)性哮喘惡化
文獻(xiàn)標(biāo)題：Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
影響因子：16.6
發(fā)表期刊：Nature communications
發(fā)表單位：上海交通大學(xué)藥學(xué)院
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Ogt shRNA干擾質(zhì)粒
原文引用：Mouse Ogt short hairpin RNA (shRNA) vector(pGMLV-SB3) was purchased from Genomeditech. Co., Ltd. (Shanghai,China), and the sequences are shown in Supplementary Table 3.
報(bào)告基因產(chǎn)品篇
4、放射治療誘導(dǎo)的星形膠質(zhì)細(xì)胞衰老促進(jìn)膠質(zhì)母細(xì)胞瘤的免疫抑制微環(huán)境，促進(jìn)腫瘤再生
文獻(xiàn)標(biāo)題：Radiotherapy-Induced Astrocyte Senescence Promotes an Immunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth
影響因子：15.1
發(fā)表期刊：Advanced Science
發(fā)表單位：浙江大學(xué)醫(yī)學(xué)院附屬第二醫(yī)院
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Myc-Luc和CXCL1啟動(dòng)子報(bào)告基因質(zhì)粒
原文引用：The Myc-Max responsive luciferase reporter plasmid (Myc-Luc reporter), and CXCL1 promoter luciferase reporter plasmids (pGL3-CXCL1-Wild-type [WT]/DEL1/DEL2) were synthesized by Genomeditech Co., Ltd(Shanghai, China).
5、Maf1 缺失可調(diào)節(jié)脊柱生成并減輕阿爾茨海默病中的認(rèn)知障礙
文獻(xiàn)標(biāo)題：Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer’s disease
影響因子：14.5
發(fā)表期刊：Brain
發(fā)表單位：同濟(jì)大學(xué)醫(yī)學(xué)院附屬上海東方醫(yī)院
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Maf 過(guò)表達(dá)質(zhì)粒和 Grin1啟動(dòng)子報(bào)告基因質(zhì)粒
原文引用：The Maf overexpressed plasmid, the wild-type Grin1 promoter luciferase (Luc) and mutant Grin1 promoter luciferase were constructed by Genomeditech Co. Ltd.
6、YAP 調(diào)控的 APIP 促進(jìn)頭頸鱗狀細(xì)胞癌中的蛋氨酸循環(huán)和轉(zhuǎn)移
文獻(xiàn)標(biāo)題：APIP regulated by YAP propels methionine cycle and metastasis in head and neck squamous cell carcinoma
影響因子：9.7
發(fā)表期刊：Cancer Letters
發(fā)表單位：上海市第九人民醫(yī)院
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APIP啟動(dòng)子報(bào)告基因質(zhì)粒
原文引用：Targeting the APIP promoter region were synthesized by Genomeditech (Shanghai, China).
7、阻斷 EGR1/TGF-β1和 CD44s/STAT3交互抑制胃癌腹膜轉(zhuǎn)移
文獻(xiàn)標(biāo)題：Blocking EGR1/TGF-β1 and CD44s/STAT3 Crosstalk Inhibits Peritoneal Metastasis of Gastric Cancer
影響因子：9.2
發(fā)表期刊：International Journal of Biological Sciences
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院瑞金醫(yī)院
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TGF-β1啟動(dòng)子報(bào)告基因質(zhì)粒和引物
原文引用：Lentiviruses were constructed with specific primers(forward: 5’-GATCCGCGCAGATCGATTTGA-3’,
reverse: 5’-AATTAAAAAAGCGCAGATCGAT-3’)from Genomeditech (Shanghai, ChinaThe wild-type TGF-β1promoter (WT, -1900 to +100 bp), EGR1 binding site 1mutated TGF-β1 promoter (MT1, -1900 to +100 bp),EGR1 binding site 2 mutated TGF-β1 promoter (MT2,-1900 to +100 bp) and binding sites truncated TGF-β1promoter (MT3, -1900 to -183 bp) were cloned into theluciferase reporter vector pGL3 basic (Genomeditech)
腺相關(guān)病毒產(chǎn)品篇
8、慢性皮炎中 20-HETE 介導(dǎo)的 TRPV1 激活通過(guò) MrgprA3+ 神經(jīng)元驅(qū)動(dòng)異動(dòng)
文獻(xiàn)標(biāo)題：20-HETE mediated TRPV1 activation drives allokinesis via MrgprA3+ neurons in chronic dermatitis
影響因子：12.4
發(fā)表期刊：Theranostics
發(fā)表單位：南京中醫(yī)藥大學(xué)
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hM4Di-mCherry 化學(xué)遺傳AAV
原文引用：To genetically silence MrgprA3 neurons using the inhibitory, Gi-coupled hM4Di DREADD (designer receptors exclusively activated by designer drugs) receptor, hM4Di-mCherry adeno-associated virus (AAV) (Genomeditech, China) was injected by tail vein (1011 VG in 100 μL) into hemizygous MrgprA3-Cre and MrgprA3.
9、吡那地爾通過(guò)抑制分子伴侶介導(dǎo)的鈣網(wǎng)蛋白自噬改善心臟微血管缺血再灌注損傷
文獻(xiàn)標(biāo)題：Pinacidil ameliorates cardiac microvascular ischemia-reperfusion injury by inhibiting chaperone-mediated autophagy of calreticulin
影響因子：9.49
發(fā)表期刊：Basic Research in Cardiology
發(fā)表單位：復(fù)旦大學(xué)附屬中山醫(yī)院
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AAV9-CRT過(guò)表達(dá)AAV
原文引用：CRT-overexpressing adeno-associated virus serotype 9 (AAV9-CRT) genomic particles were obtained from Genomeditech (Shanghai, China). To construct an endothelial cell-specific CRT overexpression mouse model, 5×1011AAV9-CRT or its negative control (AAV9-NC) genome particles was injected into 6-week-old C57BL/6 J mice via the caudal vein.
10、尼可地爾減輕糖尿病心肌病中心臟微血管鐵死亡：線粒體定位的 AMPK-Parkin-ACSL4 信號(hào)通路的作用
文獻(xiàn)標(biāo)題：Nicorandil alleviates cardiac microvascular ferroptosis in diabetic cardiomyopathy: Role of the mitochondria-localized AMPK-Parkin-ACSL4 signaling pathway
影響因子：9.3
發(fā)表期刊：Pharmacological Research
發(fā)表單位：復(fù)旦大學(xué)附屬中山醫(yī)院
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AAV9-CA-mitoAα1過(guò)表達(dá)AAV；LV-shParkin干擾慢病毒和LV-mitoACSL4、LV-mitoAα1，LV-CA-mitoAα1過(guò)表達(dá)慢病毒
原文引用：Adeno-associated virus serotype 9 (AAV9) carrying constitutively active AMPKα1 (amino acid residues from 1 to 312), containing Tie2 promoter, 3 ×Flag tag, and mitochondrial transit peptide (MTP), was defined as AAV9-CA-mitoAα1 and constructed by Genomeditech (Shanghai, China) according to methods described in a previous study.Lentiviruses containing short hairpin RNA targeting Parkin (LV-shParkin) and the negative control (LV-shNC) were constructed by Genomeditech (Shanghai, China). The LV packaging vectors of MTP-fused ACSL4 sequence (LV-mitoACSL4), MTP-fused AMPKα1 sequence (LV-mitoAα1), and MTP-fused CA-AMPKα1 sequence (LV-CA-mitoAα1) were designed by and obtained from Genomeditech (Shanghai, China)
11、丙酸通過(guò)調(diào)節(jié)背根神經(jīng)節(jié)感覺(jué) TRP 通道減輕特應(yīng)性皮炎小鼠模型的瘙癢
文獻(xiàn)標(biāo)題：Propionate alleviates itch in murine models of atopic dermatitis by modulating sensory TRP channels of dorsal root ganglion
影響因子：9.2
發(fā)表期刊：International Journal of Biological Sciences
發(fā)表單位：上海交通大學(xué)醫(yī)學(xué)院瑞金醫(yī)院
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AAV9-GPR41 干擾AAV
原文引用：The AAV9-GPR41 shRNA and control vectors were produced and purified as previously reported (Genomeditech).
慢病毒產(chǎn)品篇
12、TET2介導(dǎo)的cGAS-STING通路在肝癌細(xì)胞和內(nèi)皮細(xì)胞間交互調(diào)控血管正?；兔庖咧委?/span>
文獻(xiàn)標(biāo)題：TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
影響因子：16.6
發(fā)表期刊：Nature Communications
發(fā)表單位：海軍軍醫(yī)大學(xué)
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Tet2干擾慢病毒
原文引用：The Lentivirus-mediated shRNA expressing vectors targeting murine Tet2 (target sequence: 5′-GCTCTAAATGATGTAGCTTTG-3′) were purchased from Genomeditech (Shanghai, China) .
13、SOCS5-RBMX 刺激 SREBP1 介導(dǎo)的脂肪生成，促進(jìn)伴有 HBV 相關(guān)肝硬化的脂肪變性 HCC 的轉(zhuǎn)移
文獻(xiàn)標(biāo)題：SOCS5-RBMX stimulates SREBP1-mediated lipogenesis to promote metastasis in steatotic HCC with HBV-related cirrhosis
影響因子：7.9
發(fā)表期刊：npj Precision Oncology
發(fā)表單位：青島大學(xué)附屬醫(yī)院
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SOCS5過(guò)表達(dá)和干擾慢病毒
原文引用：Lentivirus of short hairpin RNAs (shRNAs) targeting SOCS5, SOCS5 overexpression lentivirus and the control lentivirus were purchased from Genomeditech(Shanghai, China).
14、山奈酚通過(guò) circ_0000345介導(dǎo)的 JMJD2C/β-catenin信號(hào)通路抑制大腸癌轉(zhuǎn)移
文獻(xiàn)標(biāo)題：Kaempferol inhibits colorectal cancer metastasis through circ_0000345 mediated JMJD2C/β-catenin signalling pathway
影響因子：7.9
發(fā)表期刊：Phytomedicine
發(fā)表單位：南京中醫(yī)藥大學(xué)附屬蘇州中醫(yī)醫(yī)院
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circ_0000345干擾慢病毒和對(duì)照
原文引用：Genomeditech (Shanghai, China) supplied the lenti-short hairpin-circ0000345 (sh-circ_0000345) stable lentiviral vector for circ_0000345 knockdown, along with the short-hairpin negative control (sh-NC).
15、ChemR23 信號(hào)通過(guò)抑制 NLRP3 炎性體介導(dǎo)的缺血性腦卒中神經(jīng)元焦亡來(lái)改善腦損傷
文獻(xiàn)標(biāo)題：ChemR23 signaling ameliorates brain injury via inhibiting NLRP3 inflammasome-mediated neuronal pyroptosis in ischemic stroke
影響因子：7.4
發(fā)表期刊：Journal of Translational Medicine
發(fā)表單位：上海市第六人民醫(yī)院
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ChemR23過(guò)表達(dá)慢病毒和對(duì)照
原文引用：A lentiviral vector overexpressing ChemR23 (OE) and a negative control vector (Lv-con) were purchased from Genomeditech (Shanghai, China).
16、脂肪團(tuán)和肥胖相關(guān)蛋白通過(guò) FYN/Drp1軸抑制神經(jīng)元鐵濃縮，減輕腦缺血再灌注損傷
文獻(xiàn)標(biāo)題：Fat mass and obesity associated protein inhibits neuronal ferroptosis via the FYN/Drp1 axis and alleviate cerebral ischemia/reperfusion injury
影響因子：5.5
發(fā)表期刊：CNS Neuroscience & Therapeutics
發(fā)表單位：湖南省人民醫(yī)院
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慢病毒和polybrene
原文引用：Lentivirus particles were purchased from Genomeditech (Shanghai, China).The next day, the prepared lentivirus particles were utilized for 24-h cell infection and 5?μg/mL Polybrene (GM-040901, Genomeditech).
siRNA系列
17、飛秒激光對(duì) Bmp 受體的無(wú)視蛋白激活
文獻(xiàn)標(biāo)題：Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser
影響因子：16.6
發(fā)表期刊：Nature communications
發(fā)表單位：上海交通大學(xué)生物醫(yī)學(xué)工程學(xué)院
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BMP siRNA
原文引用：Synthetic small interference RNA (siRNA) sequences targeting BMP receptors type Ia was synthesized and purchased from Genomeditech, Shanghai. siRNA with a nontargeting sequence (siNC) was used as a negative control.
18、黑色素瘤來(lái)源的外泌體通過(guò)巨噬細(xì)胞中的 IRF7/I-IFN 軸放大放療誘導(dǎo)的遠(yuǎn)隔效應(yīng)
文獻(xiàn)標(biāo)題：Melanoma Derived Exosomes Amplify Radiotherapy Induced Abscopal Effect via IRF7/I-IFN Axis in Macrophages
影響因子：15.1
發(fā)表期刊：Advanced Science
發(fā)表單位：復(fù)旦大學(xué)附屬中山醫(yī)院
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IRF7 siRNA和miR-872-3p mimics
原文引用：small interfering RNA targeting IRF7 with the sequence CCAGAUGCGUGUUCCUGUA was designed and synthesized by Genomeditech (Shanghai,China). miR-872-3p mimics and control plasmids were purchased fromGenomeditech (Shanghai, China)
19、骨細(xì)胞線粒體通過(guò)STING通路抑制癌癥轉(zhuǎn)移和發(fā)展
文獻(xiàn)標(biāo)題：Osteocyte mitochondria inhibit tumor development via STING-dependent antitumor immunity
影響因子：13.6
發(fā)表期刊：Science Advances
發(fā)表單位：浙江大學(xué)醫(yī)學(xué)院附屬第二醫(yī)院
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Cgas , Bax siRNA
原文引用：For Cgas or Bax KD, LLC or MLO-Y4 cells were transfected with Cgas small interfering RNA (siRNA), Bax siRNA, or negative control siRNA (Genomeditech Co. Ltd.) relatively using Lipofectamine 3000 transfection reagents.
試劑產(chǎn)品篇
20、金屬蛋白納米顆粒支架通過(guò)金屬增強(qiáng)的STING 激活共同遞送抗原和佐劑，用于預(yù)防病毒感染的疫苗接種
文獻(xiàn)標(biāo)題：A metalloprotein nanoparticle scaffold co-delivers antigens and adjuvants with metal-enhanced STING activation for vaccination preventing viral infections
影響因子：17.4
發(fā)表期刊：Nano Today
發(fā)表單位：天津大學(xué)
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熒光素底物
原文引用：Then 200 μL luciferase substrate (Genomeditech) was added to measure luciferase activity.
21、NKD2 控制成骨細(xì)胞和破骨細(xì)胞的分化并改善卵巢切除小鼠的骨質(zhì)流失
文獻(xiàn)標(biāo)題：Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized mice
影響因子：6.8
發(fā)表期刊：Genes & Diseases
發(fā)表單位：天津醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院
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慢病毒包裝試劑盒
原文引用：The packaging of NKD2 or control vector lentivirus was performed by transfecting the plasmids into 293T cells using a lentiviral packaging kit (Genomeditech, Shanghai, China）
22、膽汁酸通過(guò)NLRP3炎癥小體途徑誘導(dǎo)肝纖維化及FXR抑制NLRP3激活的機(jī)制
文獻(xiàn)標(biāo)題：Bile acids induce liver fibrosis through the NLRP3 inflammasome pathway and the mechanism of FXR inhibition of NLRP3 activation
影響因子：6.6
發(fā)表期刊：Hepatology International
發(fā)表單位：貴州醫(yī)科大學(xué)附屬醫(yī)院
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熒光素酶報(bào)告基因檢測(cè)試劑盒
原文引用：Twenty-four hours after transfection, the luciferase activity of firefly and Renilla was measured with a luciferase reporter assay kit (Genomeditech, Shanghai, China)
23、RUNX1靶向AKT3促進(jìn)LPS誘導(dǎo)的ARDS中肺泡高凝和纖溶抑制
文獻(xiàn)標(biāo)題：RUNX1 targeting AKT3 promotes alveolar hypercoagulation and fibrinolytic inhibition in LPS induced ARDS
影響因子：5.8
發(fā)表期刊：Respiratory Research
發(fā)表單位：貴州醫(yī)科大學(xué)附屬醫(yī)院
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HG轉(zhuǎn)染試劑和報(bào)告基因
原文引用：Next day, cells were co-transfected with the control pcDNA3.1 empty vector (EV, 250 ng) or the wild-type RUNX1 plasmid (250 ng)-based frefy luciferase reporter plus the renilla luciferase control vector pRL-TK (25 ng) using the HG transgene reagent (genomeditec, TG-10012-S). cell lysates were prepared and firely/renilla luciferase values were quantifed using Firely Luciferase Assay (Genomeditech).
