最新！二季度客戶文獻精選集錦來啦
2024年第二季度共收集引用Genomeditech Co.,Ltd. (Shangha,China)文章共計144篇，影響因子(IF)超880分。
其中包括Journal of Hepatology、Cancer Communications、Bioactive Materials、Nature communications、Advanced Science、Cell Death and Differentiation、Cell Discovery等影響因子10+以上的文章共30篇。
文章所涵蓋的服務(wù)包含過表達/干擾質(zhì)粒構(gòu)建、慢病毒、腺相關(guān)病毒AAV、CRISPR/Cas9基因敲除、細胞株構(gòu)建、熒光素酶報告基因等，此外還有熒光素底物（如鉀鹽），脂質(zhì)體轉(zhuǎn)染試劑，慢病毒包裝和濃縮試劑盒，siRNA等常用科研類試劑。
本篇精選了二季度新發(fā)的部分高質(zhì)量文章，讓我們一起追蹤最新的醫(yī)學研究進展！
慢病毒 & 細胞系列
1、EMC10 以異構(gòu)體特異性方式調(diào)節(jié)肝臟 ER 應(yīng)激和脂肪變性
文獻標題：EMC10 modulates hepatic ER stress and steatosis in an isoform-specific mann
影響因子：26.8
發(fā)表期刊：Journal of Hepatology
發(fā)表單位：復旦大學王宣春團隊
摘要
【背景介紹】內(nèi)質(zhì)網(wǎng) (ER) 膜蛋白復合物亞基 10 (EMC10) 與肥胖有關(guān)。作者研究了 EMC10 的兩種同工型，包括分泌型同工型(scEMC10)和 ER 膜結(jié)合同工型(mEMC10)，在代謝功能障礙相關(guān)的脂肪肝病 (MASLD) 中的作用。
【研究方法】采用多種脂肪變性小鼠模型和 HepG2 細胞來研究 EMC10 在肝臟 PERK-eIF2α-ATF4 信號傳導和肝脂肪變性調(diào)節(jié)中的作用。探討scEMC10中和抗體對小鼠肝脂肪變性的治療作用。
【研究結(jié)果】scEMC10 促進肝臟 PERK-eIF2α-ATF4信號傳導的激活，而mEMC10 抑制?；蚯贸觿×诵∈蟮母闻K ER 應(yīng)激和脂肪變性，而mEMC10的肝臟過度表達則受到缺乏蛋氨酸和膽堿的飲食或衣霉素的挑戰(zhàn)，這突顯了mEMC10通過調(diào)節(jié)肝臟 ER 應(yīng)激在 MASLD 中的直接抑制作用。scEMC10 的過度表達促進了脂肪肝小鼠的肝脂肪變性，而循環(huán) scEMC10 的中和則阻止了其發(fā)生，這表明scEMC10在MASLD發(fā)展中的作用。
這些發(fā)現(xiàn)證明了 EMC10 在 MASLD 發(fā)病機制中具有新的異構(gòu)體特異性作用，并通過基于抗體的中和確定了分泌的異構(gòu)體作為 MASLD 的易處理治療靶點。
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pLEX-mEMC10過表達慢病毒
2、頭頸鱗狀細胞癌的代謝景觀揭示了免疫逃逸中新的犬尿氨酸/SIGLEC-15軸
文獻標題：Metabolic landscape of head and neck squamous cell carcinoma informs a novel kynurenine/Siglec-15 axis in immune escape
影響因子：20.09
發(fā)表期刊：Cancer Communications
發(fā)表單位：上海交通大學醫(yī)學院附屬第九人民醫(yī)院
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SIGLEC15過表達慢病毒
原文引用：Mouse SIGLEC15 cDNA was cloned into the PGMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro vector (Genomeditech, Shanghai, China).
3、ETV2調(diào)節(jié)PHD2-HIF-1α軸控制代謝重編程促進血管化骨再生
文獻標題：ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regeneration
影響因子：17.99
發(fā)表期刊：Bioactive Materials
發(fā)表單位：安徽醫(yī)科大學
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EGLN1(PHD2)過表達慢病毒；雙熒光素酶報告基因
原文引用：EGLN1(PHD2)-encoding lentiviruses (Genomeditech, China) was constructed.
Target fragments were then inserted downstream of the firefly luciferase (FL) gene into the pGL3-basic luciferase reporter vector (Genomeditech) using seamless cloning
4、混合膜包覆納米顆粒用于阿爾茨海默病的精確靶向和協(xié)同治療
文獻標題：Hybrid Membrane-Coated Nanoparticles for Precise Targeting and Synergistic Therapy in Alzheimer's Disease
影響因子：14.29
發(fā)表期刊：Advanced Science
發(fā)表單位：浙江大學
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CCR2-RFP HEK293T穩(wěn)轉(zhuǎn)細胞株
原文引用：Stable CCR2-red fluorescent protein (RFP) transfected HEK293T and RFP transfected HEK293T cells were generated by Genomeditech, China.
5、靶向Rab7-Rilp介導的微脂肪自噬緩解糖尿病性心肌病中的脂質(zhì)毒性
文獻標題：Targeting Rab7-Rilp Mediated Microlipophagy Alleviates Lipid Toxicity in Diabetic Cardiomyopathy
影響因子：14.29
發(fā)表期刊：Advanced Science
發(fā)表單位：上海交通大學醫(yī)學院附屬第一人民醫(yī)院
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FLAG-Rab7 (WT), FLAG-Rab7 (S17A), FLAG-Rab7 (S72A), FLAG-Rab7 (Y183A) and HA-Rilp 過表達質(zhì)粒；RFP-GFP-LC3自噬慢病毒；GST-Rilp or Rab7Y183A過表達慢病毒
原文引用：For transfection of siRNA, HL-1 cell were seeded in a six-well plate and transfected with siRilp (Genomeditech Co., Ltd. (Shanghai, China)) and si-control according to the manufacturer's instructions. For transfection of plasmid, HEK 293T was transfected with FLAG-Rab7 (WT), FLAG-Rab7 (S17A), FLAG-Rab7 (S72A), FLAG-Rab7 (Y183A) and HA-Rilp plasmids (Genomeditech Co., Ltd. (Shanghai, China)) and vector control according to the manufacture instructions. For transfection of RFP-GFP-LC3 lentivirus, HL-1 cells were seeded in a six-well plate or 96-well plates and transfected with RFP-GFP-LC3 (Genomeditech Co., Ltd. (Shanghai, China)) following the manufacture's protocol. For transfection of lentivirus, NMCMs or HL-1 cells were seed in 6 cm dishes (for immunoprecipitation), 6-well plates (for immunoblots) or 96-well plates (for immunoflurorences) before transfecting with GST-Rilp or Rab7Y183A lentivirus following protocols from Genomeditech Co., Ltd. (Shanghai, China).
6、PAX6通過增強MET/STAT5A介導的染色質(zhì)可及性促進前列腺癌的神經(jīng)內(nèi)分泌表型
文獻標題：PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibility
影響因子：11.39
發(fā)表期刊：Journal of Experimental & Clinical Cancer Research
發(fā)表單位：上海交通大學醫(yī)學院
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human PAX6 shRNA慢病毒
原文引用：A human PAX6 lentiviral expression construct containing a puromycin resistance gene was purchased from Genomeditech PAX6 short hairpin RNA (shRNA) expression constructs were purchased from Genomeditech.
7、伴隨的 NAFLD 通過 CXCL5/CXCR2 招募結(jié)直腸癌中的 MDSC，促進肝轉(zhuǎn)移和 PD-1 難治性
文獻標題：Concomitant NAFLD Facilitates Liver Metastases and PD-1-Refractory by Recruiting MDSCs via CXCL5/CXCR2 in Colorectal Cancer
影響因子：7.1
發(fā)表期刊：Cellular and Molecular Gastroenterology and Hepatology
發(fā)表單位：山東省腫瘤醫(yī)院
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CMV-Luciferase-PGK-NEO活體成像慢病毒
原文引用：CMV-Luciferase-PGK-NEO lentivirus was purchased from Genomeditech and transfected into MC38 cells to make the cells fluorescent.
假病毒系列
8、通過分層介孔金屬有機框架佐劑增強 STING 激活來增強體液和細胞免疫
文獻標題：Boosting humoral and cellular immunity with enhanced STING activation by hierarchical mesoporous metal-organic framework adjuvants
影響因子：10.50
發(fā)表期刊：Journal of Controlled Release
發(fā)表單位：上海交通大學醫(yī)學院
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SARS-CoV-2 Spike (B.1.617.2) 假病毒
原文引用：The SARS-CoV-2 Spike (B.1.617.2) pseudotyped virus (GFP-luciferase) and GMOne-Step Luciferase Reporter Gene Assay Kit were purchased from Genomeditech Co. Ltd..
CRISPR/Cas9系列
9、源自 Akkermansia muciniphila 的十五烷酸通過調(diào)節(jié)糖酵解增強奧沙利鉑對胃癌的敏感性
文獻標題：Akkermansia muciniphila-derived pentadecanoic acid enhances oxaliplatin sensitivity in gastric cancer by modulating glycolysis
影響因子：9.1
發(fā)表期刊：Pharmacological Research
發(fā)表單位：解放軍總醫(yī)院
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FUBP1敲除單克隆
原文引用：The CRISPR/Cas9 system was used to construct FUBP1-KO GC cell lines. The FUBP1 sgRNA was designed and synthesized by Genomeditech (Shanghai, China) For RALY-overexpression cells, full-length cDNA of mouse RALY was cloned and inserted into the PGMLV-CMV-MCS-Puro plasmid (Genomeditech, Shanghai, China).
10、MAP4K4通過促進Drp1的S-亞硝基化修飾加劇糖尿病患者的心臟微血管損傷
文獻標題：MAP4K4 exacerbates cardiac microvascular injury in diabetes by facilitating S-nitrosylation modification of Drp1
影響因子：8.49
發(fā)表期刊：Cardiovascular Diabetology
發(fā)表單位：南京醫(yī)科大學
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Single-guide RNAs (sgRNAs)
原文引用：Genomic mutations were introduced into cells using the CRISPR–Cas9 system. Single-guide RNAs (sgRNAs) were designed by Genomeditech (China, Shanghai) to target the genomic area adjacent to mutation sites in C505 or C644.
腺相關(guān)病毒系列
11、腰椎不穩(wěn)定重塑軟骨終板，通過 Hippo-CCL3 信號招募破骨細胞誘導椎間盤退變
文獻標題：Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling
影響因子：14.29
發(fā)表期刊：Bone Research
發(fā)表單位：蘇州大學附屬第一醫(yī)院
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AAV5-Yap1 過表達AAV
原文引用：AAV5-Yap1 was purchased from Genomeditech.
12、LPS 誘導的單羧酸轉(zhuǎn)運蛋白 1 抑制促進乳酸積累，引發(fā)上皮間質(zhì)轉(zhuǎn)化和肺纖維化
文獻標題：LPS-induced monocarboxylate transporter-1 inhibition facilitates lactate accumulation triggering epithelial-mesenchymal transformation and pulmonary fibrosis
影響因子：6.2
發(fā)表期刊：Cellular and Molecular Life Sciences
發(fā)表單位：上海交通大學醫(yī)學院附屬仁濟醫(yī)院
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MCT1過表達AAV5；抗生素嘌呤霉素；MCT1 siRNA
原文引用：In order to establish an alveolar epithelial cells specific MCT1-overexpressing (MCT1OE) mouse model, intratracheal administration of 40 μl MCT1OE adeno-associated virus (AAV) mixture (AAV concentration: 5?×?1012 VG/ml), purchased from Genomeditech (Shanghai, China)
After 2 days, stable-transfected cells were selected with 10 μg/mL of puromycin (Genomeditech, China).
Mouse MCT1 small interfering RNA (siRNA) was purchased from Genomeditech (Shanghai, China). MCT1 siRNA
質(zhì)粒系列
13、線粒體基因組轉(zhuǎn)移驅(qū)動鄰近結(jié)腸上皮細胞的代謝重編程，促進 TGFβ1 介導的腫瘤進展
文獻標題：Mitochondrial genome transfer drives metabolic reprogramming in adjacent colonic epithelial cells promoting TGFβ1-mediated tumor progression
影響因子：14.7
發(fā)表期刊：Nature Communications
發(fā)表單位：上海交通大學醫(yī)學院附屬第一人民醫(yī)院
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TFAM, RelA, IκBα, and TGFβ1 shRNA慢病毒質(zhì)粒
原文引用：Non-target control shRNA (shNT) and shRNA targeting TFAM, RelA, IκBα, and TGFβ1 were cloned into the pGMLV-hU6 lentiviral vector (Genomeditech, Shanghai, China).
14、CCL9/CCR1 軸驅(qū)動的趨化納米囊泡通過捕獲 TGF-β 來減弱 SMAD4 缺陷型結(jié)直腸癌的轉(zhuǎn)移
文獻標題：CCL9/CCR1 axis-driven chemotactic nanovesicles for attenuating metastasis of SMAD4-deficient colorectal cancer by trapping TGF-β
影響因子：14.70
發(fā)表期刊：Acta Pharmaceutica Sinica B
發(fā)表單位：華中科技大學同濟醫(yī)學院
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CCR1 or TGFBR2過表達慢病毒質(zhì)粒
原文引用：MC38 stable cell lines were constructed by lentivirus. Plasmids encoding CCR1 or TGFBR2 (pLenti-GFP/puro-CCR1 and pLenti-RFP/puro-TGFBR2) were purchased from Genomeditech (Shanghai, China).
15、MEF2D在IL-13刺激下直接誘導H1X促進胃癌細胞肝轉(zhuǎn)移
文獻標題：MEF2D facilitates liver metastasis of gastric cancer cells through directly inducing H1X under IL-13 stimulation
影響因子：9.10
發(fā)表期刊：Cancer Letters
發(fā)表單位：浙江大學醫(yī)學院附屬第一醫(yī)院
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H1X and IL13RA1干擾和過表達質(zhì)粒；
MEF2D過表達質(zhì)粒和報告基因質(zhì)粒
原文引用：The gene knockdown and overexpression plasmids of H1X and IL13RA1 were constructed by Genomeditech Co., Ltd. (Shanghai, China)
MEF2D-overexpressed plasmid and Luciferase Reporter plasmids were constructed by Genomeditech Co.,Ltd (Shanghai, China).
siRNA系列
16、骨細胞線粒體調(diào)節(jié)經(jīng)皮層血管的血管生成
文獻標題：Osteocyte mitochondria regulate angiogenesis of transcortical vessels
影響因子：14.70
發(fā)表期刊：Nature Communications
發(fā)表單位：上海交通大學
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Mouse Slit3、Notch3、Notch4、Vegfc siRNA套餐
原文引用：To generate VegfcKD-MLO-Y4, Slit3KD-MLO-Y4, Notch3KD-MLO-Y4,Notch4KD-MLO-Y4 cells, cells were plated on 12 well plate, and incubated in 1% lipofectamine (Invitrogen, Cat. L3000015) and 50 nM siRNA (Genomeditech, Shanhai, China) in opti-MEM for 6 hours (the detailed siRNA sequences are listed in Supplementary Table 2c)
17、仿生水凝膠介導的機械免疫代謝療法，可抑制ccRCC手術(shù)后復發(fā)
文獻標題：Biomimetic Hydrogel-Mediated Mechano-Immunometabolic Therapy for Inhibition of ccRCC Recurrence After Surgery
影響因子：14.29
發(fā)表期刊：Advanced Science
發(fā)表單位：同濟大學醫(yī)學院 上海市第十人民醫(yī)
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IDO1 siRNA
原文引用：The siIDO1 (sense strand 5′-GCAAUAGUAGAUACUUACA-3′), the negative control siRNA (a scrambled sequence consisting of the sense strand 5′-UUCUCCGAACGUGUCACGUTT-3′) and the Cy3-siRNA were synthesized by Genomeditech Biotechnology Co., Ltd anti-GAPDH antibody (Genomeditech, dilution 1:1000)
試劑產(chǎn)品系列
18、苯并[a]芘的暴露誘導肝癌外泌體-環(huán)狀RNA激活肺成纖維細胞并引發(fā)器官轉(zhuǎn)移
文獻標題：Exposure of benzo[a]pyrene induces HCC exosome-circular RNA to activate lung fibroblasts and trigger organotropic metastasis
影響因子：20.09
發(fā)表期刊：Cancer Communications
發(fā)表單位：上海交通大學醫(yī)學院公共衛(wèi)生學院
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熒光素底物，鉀鹽
原文引用：intraperitoneal injection of 200 μL of D-luciferin (100 mg/kg, Genomeditech, Shanghai, China) .
19、通過分層介孔金屬有機框架佐劑增強 STING 激活來增強體液和細胞免疫
文獻標題：Boosting humoral and cellular immunity with enhanced STING activation by hierarchical mesoporous metal-organic framework adjuvants
影響因子：10.50
發(fā)表期刊：Journal of Controlled Release
發(fā)表單位：上海交通大學醫(yī)學院
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GMOne-Step 熒光素酶報告基因檢測試劑盒
原文引用：The SARS-CoV-2 Spike (B.1.617.2) pseudotyped virus (GFP-luciferase) and GMOne-Step Luciferase Reporter Gene Assay Kit were purchased from Genomeditech Co. Ltd.
20、RBBP6 通過 CPSF3 依賴性替代多腺苷酸化維持膠質(zhì)母細胞瘤干細胞
文獻標題：RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation
影響因子：12.99
發(fā)表期刊：Cell Discovery
發(fā)表單位：浙江大學生命科學學院
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慢病毒濃縮試劑盒
原文引用：lentivirus concentration kit (Genomeditech, Cat#GM-040801-100)
21、整合素αVβ3拮抗劑-c(RGDyk)肽通過抑制成骨和血管生成來減輕后縱韌帶骨化的進展
文獻標題：Integrin αVβ3 antagonist-c(RGDyk) peptide attenuates the progression of ossification of the posterior longitudinal ligament by inhibiting osteogenesis and angiogenesis
影響因子：5.9998
發(fā)表期刊：Molecular Medicine
發(fā)表單位：海軍軍醫(yī)大學（第二軍醫(yī)大學）長征醫(yī)院
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Integrin αV and Integrin β3 siRNAs和GMTrans 脂質(zhì)體轉(zhuǎn)染試劑
原文引用：The siRNAs targeting Integrin αV and Integrin β3 were designed and synthesized by Genomeditech Cor (Shanghai, China). LF transfection during osteoblastogenesis in vitro was performed on the first day after osteogenic differentiation induction. The siRNA and GMTrans Liposomal Transfection Reagent (Genomeditech, Shanghai, China)
